Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-PTGR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PTGR1 also known as Prostaglandin Reductase 1 acts as an enzyme within cellular metabolism. It functions by catalyzing the reduction of prostaglandins namely converting 15-keto-prostaglandins to their respective 1314-dihydro metabolites. PTGR1 has a molecular mass of approximately 35 kDa. This enzyme primarily expresses in liver tissues but it is present in other tissues like the lungs and kidneys as well.
Biological function summary
PTGR1 participates in metabolizing prostaglandins which play important roles in inflammation and homeostasis. It does not form part of a larger protein complex but works independently to control the levels of active prostaglandins within cells. This activity regulates physiological processes such as inflammation and vascular tone.
Pathways
Prostaglandin metabolism involves PTGR1's function. It integrates into the arachidonic acid pathway. Within this pathway PTGR1 interacts with other enzymes such as cyclooxygenases and lipoxygenases orchestrating the catabolism of prostaglandins and related lipid compounds. This coordination maintains the balance of inflammatory responses.
PTGR1 contributes significantly to inflammatory conditions and cancer. Elevated or altered PTGR1 activity associates with various inflammatory diseases due to its role in prostaglandin catabolism. Additionally in some cancer types abnormal prostaglandin levels mediated by PTGR1 affect tumor growth. The enzyme's participation in these processes links it to proteins such as COX-2 which is critical for prostaglandin synthesis and inflammation.
