Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-RCN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RCN1 also known as reticulocalbin-1 or RCN is a calcium-binding protein located in the lumen of the endoplasmic reticulum (ER). It belongs to the CREC family of proteins which are characterized by their EF-hand motifs that mediate calcium binding. RCN1 has a molecular mass of around 37 kDa. This protein is expressed in various tissues including the pancreas heart and brain suggesting its involvement in diverse cellular functions. Within the ER RCN1 interacts with other proteins aiding in their proper folding and assembly.
Biological function summary
RCN1 plays a role in cellular calcium homeostasis and may influence processes such as protein folding and trafficking. It is not known to be a part of a larger complex but it interacts with other ER-resident proteins. Maintaining calcium levels is important within the ER where RCN1 helps buffer calcium concentrations. This function supports processes that are sensitive to calcium fluctuations such as signal transduction and stress responses.
Pathways
The role of RCN1 in cellular mechanisms connects it to important pathways like the calcium signaling pathway and the unfolded protein response pathway. In the calcium signaling pathway RCN1 aids in regulating calcium levels within the ER affecting downstream signaling events. Meanwhile in the unfolded protein response pathway RCN1 participates by ensuring the proper folding of proteins particularly during times of stress when misfolded proteins accumulate. RCN1's interaction with calumenin another calcium-binding protein highlights its involvement in these pathways.
RCN1 has associations with cardiovascular disease and some forms of cancer. In cardiovascular disease altered expression of RCN1 may impact calcium signaling and lead to dysfunction in heart cells. Similarly in certain cancers changes in RCN1 expression levels could affect cellular proliferation via calcium-regulated pathways. The interaction between RCN1 and calreticulin a multifunctional protein involved in calcium homeostasis and immune response further connects RCN1's influence on disease progression.
