Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CCP110, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CP110 also known as centrosomal protein 110 is a critical component involved in centriole duplication spindle pole organization and ciliogenesis. It has a molecular weight of approximately 110 kDa. This protein is expressed in various tissues but shows higher expression in those with active cell division cycles such as embryonic tissues and cancer cell lines. As a centrosomal protein CP110 localizes to the centrosome an important cellular structure that manages microtubule organization and cell cycle progression.
Biological function summary
CP110 interacts with several other proteins to regulate centrosome function and cell division. It is a part of the centriole duplication complex which is essential for proper ciliogenesis and cell cycle control. CP110 caps the mother centriole and prevents the formation of cilia when the cell is in a dividing state therefore playing a role in the balance between cell division and the formation of cilia which contribute to cellular signaling and sensory functions.
Pathways
The centriole duplication and ciliogenesis processes involve CP110 as it interacts with pathways such as the cell cycle and the Sonic Hedgehog signaling pathway. CP110 partners with proteins like CEP97 and CEP290 to manage the length of centrioles and assist in the timely progression of the cell cycle. The regulation by CP110 ensures that centrosome duplication happens correctly preventing errors that might lead to cell division abnormalities.
CP110 has connections to ciliopathies and cancer. In ciliopathies where cilia formation is compromised impaired CP110 regulation can lead to disorders like Bardet-Biedl syndrome. In cancer overexpression or misregulation of CP110 may result in aberrant cell division and tumorigenesis. Another protein Cyclin-dependent kinase 2 (CDK2) plays a role in cell cycle regulation and works closely with CP110 indicating that disturbances in this interaction could also contribute to these diseases.
