Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-COX7A2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
COX7A2 also known as cytochrome c oxidase subunit 7A2 is a component of the cytochrome c oxidase complex found in the inner mitochondrial membrane. This protein has an approximate molecular mass of 9.8 kDa. COX7A2 is expressed in various tissues with notable expression in highly metabolic tissues such as the heart and skeletal muscle. It plays a role in the electron transport chain by participating in the reduction of oxygen to water.
Biological function summary
COX7A2 contributes to the cytochrome c oxidase (complex IV) of the mitochondrial electron transport chain. This complex is important for ATP production through oxidative phosphorylation. COX7A2 forms part of the multi-subunit complex that facilitates efficient transfer of electrons which is essential for mitochondrial energy production. Its function in this complex underlines its importance in maintaining cellular respiration and energy homeostasis.
Pathways
COX7A2 plays an important role in the mitochondrial oxidative phosphorylation pathway. This pathway is essential for ATP production in eukaryotic cells. Another significant pathway involving COX7A2 is the respiratory electron transport pathway. Within these pathways COX7A2 interacts with other cytochrome c oxidase subunits like COX4 and COX5A which help regulate electron transfer and influence overall metabolic activity.
COX7A2 alterations and dysfunctions have been associated with mitochondrial disorders and myopathies. These disorders are often characterized by impaired energy metabolism due to defective oxidative phosphorylation. Within this context changes in COX7A2 expression can influence the functions of other cytochrome c oxidase subunits potentially affecting mitochondrial function and contributing to the pathological state of diseases like mitochondrial encephalomyopathy.
