Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 7 bp deletion in exon 2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GNB4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNB4 also known as G-protein subunit beta-4 is a component of the heterotrimeric G protein complex which plays a significant role in intracellular signaling. This protein has an approximate molecular weight of 39 kDa. GNB4 is primarily expressed in the brain and also detected in various tissues contributing to its diverse functional roles. As a β-subunit GNB4 forms part of a larger complex that mediates various signal transduction processes by interacting with different receptors on the cell surface.
Biological function summary
The functionality of GNB4 involves interactions within the heterotrimeric G protein complex a critical component of signal transduction pathways. This protein acts as a modulator or adapter in transmembrane signaling systems. GNB4 pairs with gamma subunits to help regulate downstream effector proteins expanding the impact of receptor-binding events initiated by external stimuli. GNB4 influences processes like cell communication and neurotransmission making it an important factor in maintaining cellular functions in neural networks.
Pathways
GNB4 plays essential roles in G-protein coupled receptor (GPCR) signaling and other signaling pathways. In GPCR signaling GNB4 works in concert with alpha and gamma subunits to mediate signal propagation. The complex modulates cyclic AMP levels and activates phospholipase C affecting various cellular responses. GNB4 interacts closely with proteins such as GNAS and ADCY1 forming part of the intricate signaling networks that drive cellular communication and response mechanisms.
Alterations or dysfunction of GNB4 can relate to certain neuropathological conditions. Mutations in the GNB4 gene have been linked to Charcot-Marie-Tooth disease which leads to peripheral neuropathy. This condition correlates with disruptions in GNB4's role in neuron signaling pathways. Additionally GNB4 interacts with proteins like PMP22 which when mutated also associates with this neuropathic disorder highlighting its involvement in these significant health conditions.
