Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 46 bp deletion in exon4 and 79 bp deletion in exon4.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HES4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HES4 also known as Hairy and Enhancer of Split 4 is a transcription factor with a molecular mass of roughly 26 kDa. It belongs to the basic helix-loop-helix (bHLH) family and is known for its role in modulating transcriptional repression. HES4 is expressed in various tissues including neural tissues where it contributes to the regulation of cellular differentiation and development. Its expression is significant during embryonic development indicating its role in early cellular identity.
Biological function summary
The function of HES4 involves transcriptional regulation of genes important for developmental processes. It often acts as a part of a transcriptional repression complex modulating gene expression patterns necessary for cellular differentiation. HES4 plays a vital role in the Notch signaling pathway influencing the balance between proliferation and differentiation of neural progenitor cells. By functioning within this complex HES4 ensures specific transcriptional outcomes are achieved impacting cell fate decisions.
Pathways
The involvement of HES4 in the Notch signaling pathway is significant. This pathway is pivotal for controlling cell fate and lineage commitment in various cell types particularly during nervous system development. Moreover HES4's interaction with other bHLH proteins such as HES1 further integrates its role into these pathways fine-tuning the outcome of cell differentiation processes. This interaction highlights the importance of HES4 in maintaining proper cellular function and development.
Aberrations in HES4 activity have been linked to developmental disorders specifically within the nervous system such as neural tube defects. Its misregulation can impact cell differentiation processes leading to improper tissue development. Additionally alterations in HES4 have associations with certain cancers particularly those involving abnormal cell proliferation. In these contexts HES4's relationship with the Notch pathway along with factors like HES1 highlights its relevance in disease progression and potential therapeutic targets.
