Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and 29 bp deletion in exon9.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-IPPK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein Inositol (13456)-pentakisphosphate 2-kinase commonly known as IPPK or IPK1 plays an important role in the phosphorylation of inositol phosphates. IPPK catalyzes the conversion of inositol pentakisphosphate (IP5) to inositol hexakisphosphate (IP6). This protein has a molecular mass of around 44 kDa. It is prominently expressed in the brain kidney and pancreas suggesting a broad importance across different tissues.
Biological function summary
IPPK contributes to the regulation of inositol phosphate metabolism. It does not act in isolation but as part of a network involving inositol polyphosphate kinases and phosphatases that help maintain cellular processes such as signal transduction and ion homeostasis. Although IPPK doesn't form part of a larger complex its activity impacts various cell signaling pathways by altering the levels of its substrate and product.
Pathways
IPPK participates significantly in the phosphoinositide signaling pathway impacting processes such as cellular growth survival and apoptosis. This pathway also involves PI3Ks and PTEN which are critical in signal transduction. Moreover IPPK indirectly influences the synthesis of IP7 regulated by inositol hexakisphosphate kinases integrating its function into broader metabolic pathways.
IPPK has associations with pathologies like cancer and metabolic disorders. Abnormal activity or expression levels of IPPK can alter cellular signaling pathways which might lead to tumorigenesis or metabolic syndrome. It closely interacts with proteins such as mTOR highlighting its role in cancer pathways and PI3K/AKT pathway dysregulation common in metabolic disorders.
