Description
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 10 bp deletion
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-MFN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mitofusin 2 also known as MFN2 is a protein involved in the regulation of mitochondrial fusion. The MFN2 molecular weight is roughly 86 kDa. It plays an important role in connecting and merging the outer membranes of mitochondria which is vital for maintaining mitochondrial function and integrity. Mitofusin 2 proteins are expressed in many tissues but they are abundantly present in energy-demanding tissues like skeletal muscle heart and the brain.
Biological function summary
Mitofusin 2 ensures the proper distribution of mitochondria within cells and regulates mitochondrial metabolism. It is a critical component of the mitochondrial fusion machinery and works closely with its homolog Mitofusin 1 (MFN1). Together they form a complex that facilitates the physical merging of mitochondrial membranes. This process is essential for mitochondrial dynamics which include not only fusion but also fission and biogenesis.
Pathways
The protein part of the fusion machinery integrates into multiple essential biological pathways including energy metabolism and apoptosis regulation. It participates in the mitochondrial fusion pathway and the PGC-1α pathway for mitochondrial biogenesis. Mitofusin 2 interacts with proteins such as PINK1 and Parkin that are known to play roles in mitophagy a process that targets damaged mitochondria for degradation indicating its involvement in maintaining mitochondrial quality control.
Mutations in Mitofusin 2 have been linked to Charcot-Marie-Tooth disease type 2A (CMT2A) a neuropathy that affects peripheral nerves. This protein also shows connections to metabolic disorders such as obesity and type 2 diabetes. In these conditions its interaction with other proteins like OPA1 involved in mitochondrial inner membrane fusion influences mitochondrial dysfunction a recognized feature contributing to disease pathogenesis. Understanding MFN2's function and role in disease can help develop targeted therapies for these conditions.
