Description
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 100%
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ACP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
