Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 185 bp deletion in exon4 and 1 bp insertion in exon4 and 31 bp deletion in exon4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-AP1B1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AP1B1 also known as Adaptor-related protein complex 1 subunit beta-1 is involved in mechanical functions related to protein sorting and vesicle formation. It has a molecular mass of approximately 105 kDa. This protein forms part of the AP-1 complex which is present in various cell types particularly expressed in epithelial tissues. AP1B1 functions as an important component in clathrin-coated vesicle transport assisting in the selection of cargo that is transported between the trans-Golgi network and endosomes.
Biological function summary
The role of the AP1B1 extends to ensuring proper cellular trafficking maintaining cell polarity and function within epithelial cells. As part of the AP-1 complex it interacts with other subunits to facilitate the budding of vesicles a critical step in cell function and homeostasis. AP1B1's involvement in intracellular transport underlines its significance in cellular processes that require precise protein localization and distribution.
Pathways
The AP1B1 protein plays an essential role in the clathrin-mediated endocytosis and post-Golgi trafficking pathways. It operates alongside other proteins like clathrin and AP-1 γ subunit coordinating the transport of receptors and other protein complexes to their specific intracellular destinations. This molecular coordination supports processes such as receptor recycling and lysosomal targeting within the cell environment.
Errors in AP1B1 function can link to specific medical conditions such as Hermansky-Pudlak syndrome and neurodegenerative diseases. In these conditions alterations in the AP1B1 protein may disrupt normal vesicle trafficking and protein sorting processes. The disorder-related disruption illustrates how AP1B1 potentially interacting with proteins like the lysosomal-associated membrane protein 2 can impact cellular homeostasis and lead to systemic effects in tissue biology.
