Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CHCHD6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CHCHD6 also known as coiled-coil-helix-coiled-coil-helix domain containing 6 is a protein with a mass of around 31 kDa. This protein is localized in the mitochondria and is expressed across several tissues indicating its broad functional importance in cellular energy metabolism. The protein structure of CHCHD6 includes a characteristic coiled-coil-helix domain which suggests a role in protein-protein interactions potentially contributing to its mechanical functions.
Biological function summary
The coiled-coil-helix domain containing protein 6 is involved in mitochondrial function and maintenance. CHCHD6 participates in the formation of complexes within the inner mitochondrial membrane. These complexes are critical for the proper assembly and stability of mitochondrial respiratory supercomplexes which facilitate efficient electron transport and energy production. This involvement positions CHCHD6 as an important player in maintaining cellular energy dynamics and bioenergetics.
Pathways
CHCHD6 has a role in the mitochondrial electron transport chain which is important for ATP production. This protein associates with pathways governing mitochondrial dynamics and energy metabolism. CHCHD6 interacts with mitochondrial proteins like OPA1 which regulates mitochondrial fusion and cristae morphology contributing to the maintenance of respiratory chain integrity and energy output.
The dysfunction of CHCHD6 can lead to altered mitochondrial homeostasis contributing to conditions such as mitochondrial disorders and neurodegenerative diseases like Alzheimer's disease. It is associated with proteins such as the mitochondrial cytochrome C oxidase which also play a role in bioenergetic processes and can influence the pathogenesis of these diseases. Understanding CHCHD6 interactions and functions could provide insights into potential therapeutic targets for these conditions.
