Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FIBP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FIBP also known as FGF-binding protein is a protein with a molecular mass of approximately 42 kDa. This protein interacts mechanically with fibroblast growth factors (FGFs) and affects their activity. FIBP is expressed in various tissues including brain heart and kidney. Researchers have studied how it binds to acidic and basic FGFs which play roles in multiple cellular processes.
Biological function summary
FIBP influences the modulation of cell growth and differentiation. It does not form a known complex but interacts with FGFs impacting their bioavailability and function. These roles make it integral to cellular processes where FGF signaling is important such as tissue repair and embryonic development. Understanding its interaction with FGFs helps in studying various physiological and pathological states.
Pathways
FIBP plays a role in the fibroblast growth factor signaling pathway. This pathway involves FGFRs (fibroblast growth factor receptors) and proteins such as ERK1/2 which are essential for signal transduction. FIBP affects FGF localization and modulation influencing how signals propagate within the pathway. This regulation holds importance in cellular proliferation and survival key functions within the growth factor signaling networks.
FIBP has associations with cancer and developmental disorders. Abnormal FGF signaling mediated by FIBP can lead to tumorigenesis and aberrant development. FGF interactions connect FIBP with FGFR mutations or dysregulations often seen in various cancers. Understanding the relationship between FIBP and disease pathways advances research on therapeutic targets and diagnostic markers.
