Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-IDH3G, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The IDH3G protein also called isocitrate dehydrogenase 3 (NAD+) gamma is a subunit of the IDH3 enzyme complex. This protein has a molecular mass of approximately 39 kDa. It is highly expressed in tissues with a high energy demand like muscle cells and neurons. IDH3G plays an important role in the mitochondrial matrix where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate producing NADH and CO2 as byproducts.
Biological function summary
IDH3G is part of the IDH3 enzyme complex which contains multiple subunits and functions within mitochondria. This complex plays a critical role in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. This cycle is fundamental for energy production as it supplies reducing equivalents in the form of NADH which drive ATP synthesis through oxidative phosphorylation. The proper functioning of IDH3G contributes to efficient cellular respiration and energy homeostasis.
Pathways
The function of IDH3G connects to the TCA cycle and the energy metabolism pathway. Within the TCA cycle IDH3G's enzymatic activity ensures the continuation of the cycle and maintains the production of NADH. This is key for the generation of ATP in oxidative phosphorylation. The IDH3 complex works closely with the other components of the TCA cycle including succinate dehydrogenase and citrate synthase ensuring the continuity and regulation of metabolic flux in energy production pathways.
Defects or alterations in IDH3G are associated with metabolic disorders such as lactic acidosis and mitochondrial diseases. These conditions can result from impaired energy production and accumulation of metabolic intermediates. Furthermore IDH3G connections with related proteins could affect cell proliferation and survival in neurodegenerative diseases. Proper regulation and function of IDH3G may impact these diseases through its influence on energy metabolism pathways.
