Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 6.25 ng/mL,
Range:6.25 - 400 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
BSA ELISA Kit ab285245 is a sandwich ELISA to measure BSA in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids with a sensitivity of 6.25 ng/ml.
How the assay works
The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
BSA ELISA Kit ab285245 protocol summary
1. Add standards or samples to appropriate wells. Incubate
2. Wash
3. Add HRP conjugate reagent to all wells. Incubate
4. Wash
5. Add Chromogen solutions to each well. Incubate.
6. Add stop solution and read at 450 nm.
This product is manufactured by BioVision, an Abcam company and was previously called E4466 QuickDetect™ BSA (Human) ELISA Kit. E4466-100 is the same size as the 96 test size of ab285245.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bovine serum albumin (BSA) also known as albumin bovine serum boasts a molecular weight of approximately 66.5 kDa. This protein is found in the blood serum of cows and is derived from plasma. BSA or bovine albumin acts as a transport protein that binds to various substances including fatty acids and hormones facilitating their movement through the blood. In the lab scientists often use the BSA protein as a standard or blocking agent in experiments particularly in ELISA and Western blot assays.
Biological function summary
Proteins such as BSA play a critical role in maintaining osmotic pressure and pH balance in biological fluids. BSA does not form part of a larger protein complex but interacts with numerous small molecules and ions stabilizing their concentrations in the bloodstream. The interaction of albumin bovine serum with fatty acids is especially important for modulating the bioavailability of lipophilic molecules.
Pathways
Albumin actively contributes to lipid metabolism and transport pathways. It interacts closely with other proteins involved in these processes such as lipoproteins. BSA's binding affinity for various drugs and toxins also places it within the detoxification and excretory pathways ensuring the stability and solubility of these substances in circulation.
Scientists have studied BSA for its relevance to liver and kidney diseases given its role in carrying molecules and maintaining osmotic balance. Reduced levels of bovine albumin may indicate liver dysfunction or nephrotic syndrome where protein loss through urine is a concern. BSA's interaction with proteins such as globulin is also significant when investigating these conditions as the balance between these proteins can reflect the body's overall protein status.
