Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.813 pg/mL,
Range:4.688 - 300 pg/mL,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
Human Amyloid Beta 42 ELISA Kit ab289832 is a sandwich ELISA to measure Human Amyloid Beta 42 in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 2.813 pg/ml.
How the assay works
The density of color is proportional to the amount of human Amyloid Beta 42 captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Amyloid Beta 42 ELISA Kit ab289832 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Amyloid beta 42 also known as Aβ42 is a peptide fragment generated through the proteolytic processing of the amyloid precursor protein (APP) by enzymes called beta-secretase and gamma-secretase. The mass of Aβ42 is approximately 4.5 kDa. It is primarily expressed in the central nervous system specifically in the brain where it plays a role in the normal synaptic function. Although in low concentrations Amyloid beta 42 is involved in a variety of neural activities.
Biological function summary
Amyloid beta 42 is an important component in the formation of amyloid plaques which are aggregates that occur extracellularly in the brain. These plaques are a major hallmark of Alzheimer's disease but generally Amyloid beta 42 can be found in both healthy and diseased brains. It is not part of a stable complex but it interacts with other types of amyloid beta peptides and possibly other molecular structures within the brain.
Pathways
The production of Amyloid beta 42 is a central event in the amyloidogenic pathway which contributes to Alzheimer's disease pathogenesis. This pathway involves the sequential action of beta-secretase and gamma-secretase on APP. Aβ42 closely interacts with proteins like presenilin which are important components of the gamma-secretase complex influencing the proteolytic cleavage that determines the length of the amyloid beta peptides produced.
Amyloid beta 42 is most notably linked to Alzheimer's disease where it contributes to neuronal death and cognitive decline along with its strong association with amyloid plaques. Some evidence also indicates its involvement in other neurodegenerative diseases like cerebral amyloid angiopathy. In Alzheimer's disease high levels of Amyloid beta 42 correlate with hyperphosphorylation of tau proteins another neuropathological feature which further drives the neurodegenerative process.
