Description
Key facts
Sample types:Cell culture media
Product details:
Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. It gives highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours with a quick protocol for routine screening. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this kit a convenient tool for routine examination of cell cultures and media.
Mycoplasma PCR Detection Kit protocol summary
- Prepare the PCR reagents and premix solution
- Add the sample to the PCR reaction mix.
- Perform the PCR cycling
Analyze the PCR products using agarose gel electrophoresis. A band at ~500bp indicates mycoplasma contamination.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mycoplasma are a group of bacteria without a cell wall which makes them unique and adaptable to various environments. Known for their small size they have a low genomic mass. They parasitize the cells of a host organism relying on host cells for nutrients and energy. Mycoplasma can express themselves in human animal and plant hosts often found in cultures used in research laboratories. Due to their lack of cell walls they can take on various shapes which complicates their detection and monitoring in lab settings.
Biological function summary
The infection caused by these bacteria can disrupt normal cellular processes of the host. Their evasion of the immune system occurs through frequent genetic changes leading to persistent infections. Mycoplasma are not part of a complex; however they interact closely with cellular structures of the host leading researchers to employ techniques like 'mycoplasma detection kit' or 'mycoplasma PCR test' to identify contamination. These methods are necessary due to the bacteria's ability to impact cellular morphology and function.
Pathways
Various processes are disrupted upon infection by these bacteria affecting essential pathways such as DNA replication and signal transduction. For example they interfere with host cell functions through interactions with proteins like toll-like receptors which are part of the host's immune response. Mycoplasma bacteria may directly alter the metabolic flow within the pathways influencing cellular productivity and viability. The 'mycoplasma testing kit' becomes valuable in identifying these bacteria ensuring accurate experimental outcomes.
Mycoplasma bacteria are associated with respiratory infections and urogenital tract disorders. In humans Mycoplasma pneumoniae causes atypical pneumonia while Mycoplasma genitalium is linked to urogenital tract infections. Their presence in cell cultures necessitates the use of tools such as the 'mycoplasma PCR kit' to prevent compromised research. These bacteria are connected to disease processes through interactions with proteins involved in the host immune response posing challenges in treatment and necessitating continued scientific investigation.
