Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Saliva, Cell culture media, Cell culture supernatant, Serum, Cell Lysate,
Reacts with:Mammals,
Assay type:Competitive,
Assay time:3h,
Assay Platform:Microplate
Product details:
cAMP Assay Kit (Competitive ELISA) ab290713 is a competitive ELISA to measure cAMP in cells and tissue treated with 0.1M HCL, plasma, saliva, serum and cell culture supernatant..
How the assay works:
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cAMP antigen and a polyclonal rabbit antibody specific to cAMP. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cAMP captured in the plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
cAMP Assay Kit (Competitive ELISA) ab290713 protocol summary
1. Add standards and samples to appropriate wells
2. Add prepared labeled AP-conjugate to appropriate wells
3. Add Cyclic AMP Complete antibody to appropriate wells
4. Incubate at room temperature
5. Add pNpp substrate to each well. Incubate at room temperature
6. Add Stop Solution to each well. Read immediately.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cyclic adenosine monophosphate (cAMP) serves as an essential secondary messenger in various signal transduction pathways. Known also by its chemical abbreviation cAMP this small molecule has a mass of approximately 329.21 g/mol. cAMP is synthesized from ATP by the enzyme adenylate cyclase which is present in many cell types notably in neurons and various endocrine tissues. cAMP acts by activating protein kinase A (PKA) which then phosphorylates target proteins to alter cellular function.
Biological function summary
CAMP regulates numerous cellular processes including metabolism gene expression and cell growth. It functions as an important part of G protein-coupled receptor (GPCR) signaling pathways where its concentration can significantly change influenced by external signals. cAMP modulates the activity of ion channels and various enzymes often participating in feedback loops to maintain cellular homeostasis.
Pathways
CAMP integrates into critical signal transduction pathways such as the cyclic AMP-dependent pathway and the calcium signaling pathway. It works closely with proteins like CREB (cAMP response element-binding protein) to regulate gene transcription. cAMP also impacts other molecules like phosphodiesterases which break it down maintaining its transient nature in response to stimuli.
Irregular cAMP levels associate with pathologies like heart failure and certain types of cancer. Disruptions in cAMP signaling can lead to abnormal cell growth or inadequate cellular response to hormonal signals. Through its interaction with proteins like CFTR (cystic fibrosis transmembrane conductance regulator) cAMP influences disorders such as cystic fibrosis highlighting its critical role in maintaining cellular function and signaling balance.
