Description
Key facts
Detection method:Colorimetric,
Sample types:Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 46.5 pg/mL,
Range:156.3 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Beta Arrestin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beta Arrestin 1 protein in Serum, Cit plasma, EDTA Plasma, Hep Plasma, and Cell culture supernatant. Quantitate Human Beta Arrestin 1 with 46.5 pg/ml sensitivity.
SimpleStep ELISA
technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details. Our SimpleStep ELISA
technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta Arrestin 1 also known as β-arrestin b-arrestin or ARRB1 is a protein that plays an important role in cell signaling mechanisms. It has a molecular mass of approximately 47 kDa and is expressed in various tissues with high expression levels in the brain and testes. Beta Arrestin 1 binds to G protein-coupled receptors (GPCRs) leading to the termination of coupling between the GPCRs and G proteins. This function is essential in regulating the receptor's signal and mediating receptor desensitization.
Biological function summary
Beta Arrestin 1 functions beyond GPCR desensitization by participating in receptor internalization and the initiation of alternative signaling pathways known as arrestin-mediated signaling. It acts as part of a greater complex with clathrin and adaptin which facilitates the endocytosis of GPCRs. In doing so beta Arrestin 1 can modulate different signaling pathways independently of G proteins influencing various cellular responses.
Pathways
Beta Arrestin 1 integrates into the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for regulating diverse cellular processes including proliferation differentiation and survival. Through the MAPK/ERK pathway beta Arrestin 1 is connected to proteins such as extracellular signal-regulated kinases (ERK1/2). In the context of the PI3K/AKT pathway it associates with phosphoinositide 3-kinase helping to control cell growth and survival mechanisms.
Disruptions involving beta Arrestin 1 have implications in cancer and cardiovascular diseases. In cancer abnormal beta Arrestin 1 activity can influence tumor growth by altering cell proliferation and survival signaling pathways. It links to proteins like ERK1/2 and PI3K which can drive oncogenic processes. In cardiovascular disorders beta Arrestin 1 is implicated in heart failure where its altered activity affects cardiovascular signaling pathways influencing heart function and response to stress.
