Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Quantitative,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Uric Acid Assay Kit (ab65344) provides a convenient means for detecting uric acid in biological samples such as serum and urine. Pretreatment of samples is not required.
Uric acid level can be measured using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ= 570 nm) methods.
Uric acid assay protocol summary:
- add reaction mix to samples and standards
- incubate for 30 min
- analyze on microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K608 Uric Acid Colorimetric/Fluorometric Assay Kit. K608-100 is the same size as the 100 test size of ab65344.
Uric acid in serum is the end product of purine metabolism, and is cleared through the kidney by glomerular filtration. However, human often lacks the necessary enzyme called urate oxidase (Uricase), and therefore abnormal uric acid may be accumulated in blood. Recent evidences show the close association between serum urate level and cardiovascular morbidity and mortality, especially among persons at high cardiovascular risk, including those with hypertension, diabetes and congestion heart failure.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Uric acid a compound with a molecular mass of approximately 168.11 g/mol is an end product of purine metabolism in humans and other primates. It forms primarily in the liver and is then transported via the bloodstream to the kidneys where it is excreted in urine. This small nitrogenous waste product serves as a marker of kidney function and metabolic health but it is not a protein. Uric acid exists in higher concentrations in the blood and urine and can crystallize when concentrations exceed its solubility leading to disorders such as gout.
Biological function summary
Uric acid plays a negligible role outside its status as a metabolic product. It does not participate in any known physiological complexes but serves as an antioxidant in the bloodstream neutralizing free radicals and potentially preventing cellular damage. Despite this its protective benefits are overshadowed by its tendency to cause issues when accumulated in high concentrations forming crystals in joints and tissues.
Pathways
Uric acid creation involves the degradation of purine nucleotides through a multi-step enzymatic process. Xanthine oxidase an important enzyme in these pathways converts hypoxanthine to xanthine and then to uric acid. This pathway overlaps with the salvage pathway which includes the enzyme HGPRT (hypoxanthine-guanine phosphoribosyltransferase) involved in recycling purines highlighting the balance between synthesis and degradation of nucleotides.
Elevated levels of uric acid are connected to gout a type of inflammatory arthritis characterized by sudden severe pain redness and swelling in the joints. This condition arises from the deposition of monosodium urate crystals particularly in joints and surrounding tissues. Uric acid levels also link to kidney stones where excess urate accumulates leading to renal complications. Proteins such as ABCG2 (ATP-binding cassette sub-family G member 2) influence the excretion of uric acid and mutations in these proteins can contribute to hyperuricemia and related disorders.
