Agilent, 200314, XL10-Gold Ultracompetent Cells, 5 x 100 ul
Catalog Number: 200314
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Category
Pack Size5 x 100 µL
| Verified Reactivity | Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | HTLV-1-transformed Lewis-S1 rat T cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310). |
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| Application References(PubMed link indicates BioLegend citation) | Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)Bluestone JA. 1995. Immunity 2:555. (IP, Block)Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)Donius LR, et al. 2014. Immunobiology. 219:440. PubMed |
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| Product Citations | He J, et al. 2023. Biomolecules. 13:. PubMed Shen W, et al. 2023. Dis Model Mech. 16: . PubMed Liu C, et al. 2022. J Inflamm Res. 15:2803. PubMed Donius L, et al. 2014. Immunobiology. 219:440. PubMed |
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| RRID | AB_2075123 (BioLegend Cat. No. 200314) |
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| Structure | Ig superfamily, 80 kD |
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| Distribution | Antigen presenting cells, activated B cells and T cells |
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| Function | T and B cell costimulation |
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| Ligand/Receptor | CD28, CD152 (CTLA-4) |
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| Cell Type | B cells, T cells, Antigen-presenting cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
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| Antigen References | 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29. |
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| Gene ID | 56822 |
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| UniProt | View information about CD86 on UniProt.org |
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| Clone | 24F |
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| Regulatory Status | RUO |
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| Other Names | B7-2, B70 |
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| Isotype | Mouse IgG1, κ |
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