Agilent, 200315, XL10-Gold Ultracompetent Cells, 10 x 100 ul
Catalog Number: 200315
Send an RFQ.
Category
Pack Size10 x 100 µL
| Verified Reactivity | Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | HTLV-1-transformed Lewis-S1 rat T cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | 0.2 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310). |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application References(PubMed link indicates BioLegend citation) | Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block) Bluestone JA. 1995. Immunity 2:555. (IP, Block) Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC) Donius LR, et al. 2014. Immunobiology. 219:440. PubMed |
|---|
| Product Citations | Pinho AG, et al. 2023. Int J Mol Sci. 24:. PubMed |
|---|
| RRID | AB_2910355 (BioLegend Cat. No. 200315) AB_2910355 (BioLegend Cat. No. 200316) |
|---|
| Structure | Ig superfamily, 80 kD |
|---|
| Distribution | Antigen presenting cells, activated B cells and T cells |
|---|
| Function | T and B cell costimulation |
|---|
| Ligand/Receptor | CD28, CD152 (CTLA-4) |
|---|
| Cell Type | Antigen-presenting cells, B cells, T cells |
|---|
| Biology Area | Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
|---|
| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
|---|
| Antigen References | Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. Hathcock KS, et al. 1993. Science. 262:905-7. Freeman GJ, et al. 1993. Science 262:907-9. Carreno BM, et al. 2002. Ann Rev Immunol. 20:29-53. |
|---|
| Gene ID | 56822 |
|---|
| UniProt | View information about CD86 on UniProt.org |
|---|
| Clone | 24F |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | B7-2, B70 |
|---|
| Isotype | Mouse IgG1, κ |
|---|