Agilent, 600191, Pfu DNA Ligase
Catalog Number: 600191
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Category
Pack Size400 units / pack
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| Concentration | 4 U/µL |
|---|---|
| Inactivation | Must be removed by protein extraction methods |
| Ligase Type | Pfu DNA ligase |
| Quantity per Kit | 400 U |
| Reaction Conditions | 4 U of Pfu DNA ligase enzyme, in 20 mM Tris-HCl (pH 7.5), 20 mM KCl, 10 mM MgCl2, 0.1 % Igepal, 0.01 mM rATP, 1 mM DTT |
| Reactions per Kit | 400 units |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 50 % glycerol, 1 mM EDTA, 10 mM DTT, 0.1 % Tween 20, 0.1 % Igepal |
| Unit Definition | One unit will ligate to completion 0.5 µg of nicked pBluescript DNA in 15 minutes at 55°C. |
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