| Brand | ZORBAX |
|---|---|
| Hardware | SS |
| Inner Diameter (ID) | 4.6 mm |
| LC Platform | Stainless Steel |
| Length | 250 mm |
| Maximum Temperature | 80 °C |
| Part Number Application Domain | Biopharma |
| Part Number Application|MultiLine | Peptides|Proteins| |
| Particle Size | 5 µm |
| Particle Type | Fully Porous |
| Phase | SCX |
| Pore Size | 300 Å |
| Pressure Rating | 350 bar |
| Separation Mode | Ion Exchange (IEX) |
| Shipping Solvent | Methanol |
| Technique | LC |
| UNSPSC Code | 41115711 |
| pH Range | 2-7 |
Answer:It uses strong cation-exchange interactions to separate positively charged analytes.
Answer:The large pores improve access to the stationary phase for peptides and proteins.
Answer:The column is specified for mobile phases in the pH 2–7 range.
Agilent ZORBAX 300Å SCX is a strong cation-exchange HPLC column for charge-based separation of peptides, proteins, and other positively charged analytes. The 250 × 4.6 mm stainless-steel column contains 5 µm silica particles with a 300 Å pore structure that accommodates biomolecules larger than those typically targeted by small-pore reversed-phase packings. Its SCX stationary phase retains analytes through ionic interactions rather than conventional hydrophobic C18 retention. Buffer pH and ionic strength control selectivity and elution in ion-exchange workflows. The column has a 350 bar pressure rating, a specified pH range of 2–7, and a maximum temperature of 80 °C.
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