Agilent, GKE-5011A, AdvanceBio PNGase A (almond), 2 mU

Name: AdvanceBio PNGase A (almond), 2 mU
Brand: Agilent
SKU: GKE-5011A

AdvanceBio PNGase A (almond), 2 mU

Catalog Number: GKE-5011A

Pricing

Enzyme Formulation	Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source	Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.
Enzyme Specific Activity	> 5 units/mg
Enzyme Specificity	PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.

Ordering

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.

More from Agilent.