Agilent, GX24-201PC, AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 24-ct

Name: AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 24-ct
Brand: Agilent
SKU: GX24-201PC

AdvanceBio Gly-X Deglycosylation and Ins

Catalog Number: GX24-201PC

Pricing

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Category

Protein Labeling Kits

Pack Size201 pcs

CollectionsAgilent HPLC and UHPLC Columns for Small Molecule Separations Agilent J&W GC Columns Agilent Biopharma HPLC Analysis

Description

AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 24-ct. Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution phase, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GX24-100 Gly-X Deglycosylation Module, GX24-101 Gly-X InstantPC Labeling Module. For cleanup use GX96-102 Gly-X InstantPC cleanup module.

Specifications

General

Application\|MultiLine	Flexible, high-throughput format: process 1 to 24 samples\|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.\|Small molecule size for improved chromatographic peak resolution\|
Count	24
Deglycosylation Efficiency	Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %
Equipment & Reagents Provided by User\|MultiLine	96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C\|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks\|THERM-1000, 230V)\|Thermocycler (Corning THERM-1001, 110V\|
Kit Configuration	Sample Prep Module Set
Label	InstantPC, Precision (well-to-well
day-to-day)	<5% CV
Prep time (approx)	42 min (16 samples), 99 min (96 samples)
Preservation of Sialylated Species	> 95 %
Protein Load Range	1-40 µg
Recovery	> 95 %
Stability	Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF

Ordering

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