Agilent, PL2083-3001, PEO, nominal Mp 50,000 Da

The Agilent Polyethylene Oxide GPC/SEC Standard, nominal Mp 50,000 Da, is a 1 g polymer calibration reference for size-exclusion analysis of water-soluble macromolecules. In an aqueous SEC method, it provides a higher molecular-size reference point than the 20,000 and 30,000 Da PEO grades. The resulting calibration is based on polymer elution volume and can be used to estimate the apparent molecular-weight distribution of unknown polymers under comparable chromatographic conditions. Molecular conformation, solvent quality and column interactions affect this relationship, so a polymer standard should be used within a validated method rather than as an absolute mass measurement. Dissolve the reference gently in clean compatible solvent, keep particles out of the column and avoid sample concentrations that distort peak shape. Integrate the resulting chromatogram consistently with the analytical calibration procedure. This is an individual solid PEO reference, not a blended multiweight kit, and its nominal peak value is separate from weight-average molecular weight.