ACETACE-222-2504, Avantor® ACE® C8-300, HPLC Columns, 5 µm

ACE C8-300 Analytical HPLC Column is a wide-pore reversed-phase silica column developed for chromatographic separation of peptides, proteins and other analytes that benefit from reduced hydrophobic retention. The stationary phase combines C8 octyl surface chemistry with 300 Å pores, making larger biomolecules more accessible to the bonded phase than in many conventional small-pore analytical columns. This variant has 5 µm particles, a 250 mm length and a 4.0 mm internal diameter. A C8 phase generally provides less hydrophobic retention than a comparable C18 phase, but actual selectivity depends on the analyte, mobile phase and gradient. Develop methods using a suitable aqueous-organic solvent system and verify that pH, temperature and pressure remain within the column limits. Condition the column before injections and minimize particulate contamination using appropriate sample preparation. Monitor peak symmetry, retention stability and pressure over repeated runs. The product is a complete analytical HPLC column, not bulk C8 stationary phase or a preparative column. Column dimensions should be matched carefully when transferring a validated method.