BD, 550333, Biotin Rat Anti-Mouse IgG2b R12-3

The R12-3 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.
This antibody conjugate has been tested as a detecting reagent in ELISA (~2 µg/ml) to assure purity and reactivity. For immunohistochemical staining, a dilution range of 1:25 - 1:100 is suggested on acetone-fixed frozen sections.
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.