BD, 553986, Purified Rat IgG2b, κ Isotype Control A95-1

The A95-1 antibody has unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten that is not expressed on human, mouse, rat, or non-human primate cells. The A95-1 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
Immunocytochemistry: Purified Rat IgG2b, κ Isotype Control (Cat. No. 559478) is an immunoglobulin isotype control for rat IgG2b and can be used to determine the level of non-specific background staining in an indirect cytokine immunocytochemical assay. Purified Rat IgG2b, κ Isotype Control antibody should be utilized at the same concentration as the primary specific antibody and visualized under the same conditions via a three step staining procedure in combination with Biotin Goat Anti-Rat IgG (Cat. No. 559286) followed by Streptavidin HRP (Cat. No. 550946) and DAB substrate kit (Cat. No. 550880). Please see protocol for a detailed description of the immunocytochemical procedure at: www.bdbiosciences.com/us/s/resources.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.