The monoclonal antibody 6D4/D6/G2 reacts with human CXC chemokine, interferon gamma inducible protein 10 (IP-10). IP-10 is inducible in monocytes, keratinocytes and endothelial cells by IFN-γ. The immunogen used to generate the monoclonal antibody 6D4/D6/G2 was recombinant human IP-10 protein.
ELISA: Biotin Mouse Anti-Human IP-10 (Cat. No. 555048) should be titrated to determine its optimal concentration for ELISA detection (0.5 - 2.0 µg/ml). To obtain linear standard curves, doubling dilutions of Recombinant Human IP-10 (Cat. No. 551130) ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com/us/s/resources, and go to the protocols section under ELISA and ELISPOT. Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, lymphotactin, MIG, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TGF-β, TNF, LT-α and mouse Crg-2). Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human IP-10 in serum or plasma our Human IP-10 BD OptEIA™ ELISA Set (Cat. No. 550926) is specially formulated and recommended. Immunofluorescent Staining and Flow Cytometric Analysis: The 6D4/D6/G2 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate human IP-10 producing cells within mixed cell populations. The PE-conjugated 6D4/D6/G2 antibody (Cat. No. 555049) is suitable for these experiments. For specific methodology, please visit our web site, www.bdbiosciences.com/us/s/resources, and go to the protocols section under Cell Biology (WB, IP, IHC, IF) or Cytokines (Intracellular Staining).
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
