BD, 559626, HRP Rat Anti-Mouse IgG1 X56

The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.
Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000. For sandwich ELISA HRP Rat Anti-Mouse IgG1 is optimal for detection with Purified Rat Anti-Mouse IgG1 (Cat. No. 553445) for capture. Warning: HRP Rat Anti-Mouse IgG1 contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1). Hazard statements May cause an allergic skin reaction. Precautionary statements Wear protective gloves / eye protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Dispose of contents/container in accordance with local/regional/national/international regulations.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. ProClin is a trademark of Rohm and Haas Company.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.