BD, 611306, Purified Mouse Anti-IKKγ 54/IKKγ/NEMO

In most cells, NF-κB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IκBα, IκBβ, and IκBε. Cell stimulation induces the release, activation, and nuclear translocation of NF-κB. Release of NF-κB results from the phosphorylation and subsequent proteolytic degradation of the IκB proteins. Two cytokine-inducible IκB kinases (IKKα and IKKβ) phosphorylate and target the IκB proteins for degradation by the ubiquitin pathway. These kinases are components of a 700-900 kDa multisubunit complex that also contains NF-κB/RelA, IκBα, MEKK1, NIK, IKAP, and IKKγ/NEMO (NF-κB essential modulator). IKKγ contains two coiled-coil domains and a leucine zipper which allow it to form dimers and trimers that interact directly with IKKβ. IKKγ, essential for IKKα/IKKβ activation of NF-κB, is located functionally and physically upstream of these subunits. In addition, IKKγ enables the HTLV-1 Tax oncoprotein to interact with IKKβ, resulting in constitutive activation of NF-κB and maintenance of cellular transformation. Thus, IKKγ is an essential element of the IκB complex and an adaptor that mediates stable formation of Tax-IKK complexes.
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.