Beckman Coulter, 303402, 13mm Diameter Delrin Tube Adapter, Quantity of One
Catalog Number: 303402
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Category
| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human myeloid leukaemia cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified ICC, IP, WB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438). |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF) |
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| Product Citations | Consonni FM, et al. 2023. Front Immunol. 14:1168455. PubMed Alpert A, et al. 2022. Cell Syst. 13:71. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Wen J, et al. 2020. J Pathol Clin Res. 0.36875. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Zhang Y, et al. 2020. Int J Mol Sci. 21:00. PubMed Zhang Y, et al. 2022. Exp Hematol Oncol. 11:15. PubMed Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed Henrick BM, et al. 2021. Cell. . PubMed Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Hou Z, et al. 2020. Oncol Lett. 19:3982. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed |
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| RRID | AB_314346 (BioLegend Cat. No. 303402) |
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| Structure | Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD |
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| Distribution | Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells |
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| Function | Adhesion and lectin activity |
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| Ligand/Receptor | Sugar chains containing sialic acid |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules, Siglec Molecules |
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| Antigen References | 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005. |
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| Gene ID | 945 |
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| UniProt | View information about CD33 on UniProt.org |
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| Clone | WM53 |
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| Regulatory Status | RUO |
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| Workshop | IV M-505 |
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| Other Names | Siglec-3, gp67, p67 |
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| Isotype | Mouse IgG1, κ |
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