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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human myeloid leukaemia cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438). |
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| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF) |
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| Product Citations | Fox-Fisher I, et al. 2022. Med (N Y). 3:468. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Cheung P, et al. 2018. Cell. 173:1385. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed |
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| RRID | AB_2562818 (BioLegend Cat. No. 303419) |
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| Structure | Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD |
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| Distribution | Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells |
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| Function | Adhesion and lectin activity |
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| Ligand/Receptor | Sugar chains containing sialic acid |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules, Siglec Molecules |
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| Antigen References | 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005. |
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| Gene ID | 945 |
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| UniProt | View information about CD33 on UniProt.org |
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| Clone | WM53 |
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| Regulatory Status | RUO |
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| Workshop | IV M-505 |
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| Other Names | Siglec-3, gp67, p67 |
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| Isotype | Mouse IgG1, κ |
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