Beckman Coulter, 303459, 13mm Diameter Delrin Tube Adapter, Quantity of One
Catalog Number: 303459
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human myeloid leukaemia cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438). |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF) |
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| RRID | AB_3698957 (BioLegend Cat. No. 303459) AB_3698957 (BioLegend Cat. No. 303460) |
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| Structure | Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD |
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| Distribution | Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells |
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| Function | Adhesion and lectin activity |
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| Ligand/Receptor | Sugar chains containing sialic acid |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules, Siglec Molecules |
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| Antigen References | 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005. |
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| Gene ID | 945 |
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| UniProt | View information about CD33 on UniProt.org |
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| Clone | WM53 |
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| Regulatory Status | RUO |
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| Workshop | IV M-505 |
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| Other Names | Siglec-3, gp67, p67 |
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| Isotype | Mouse IgG1, κ |
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