Beckman Coulter, 355603, 10.4 mL Polycarbonate Bottle with Cap Assembly, 16 x 76mm - 6Pk
Catalog Number: 355603
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Category
Pack Size10.4 mL
| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Jurkat cells transfected with MUC1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Application Notes |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1. |
| Application References(PubMed link indicates BioLegend citation) | Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed |
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| Product Citations | Lee JK, et al. 2015. Front Cell Dev Biol. 3:13. PubMed Kaspi E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183136. PubMed Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed Nyeng P, et al. 2020. Developmental Cell. 49(1):31-47. PubMed Jónsdóttir HR, et al. 2019. Viruses. 11:747. PubMed Song W, et al. 2012. Int J Oncol. 41:1977. PubMed |
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| RRID | AB_2561643 (BioLegend Cat. No. 355603) AB_2561644 (BioLegend Cat. No. 355604) |
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| Structure | 500-1000 kD proteoglycan (glycosylated form) |
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| Distribution | Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers |
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| Interaction | Complexes with SOS1 and Grb2 in RAS signaling |
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| Ligand/Receptor | ICAM-1 and Her2/neu (ErbB2) |
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| Cell Type | Dendritic cells, Epithelial cells, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339. 2. Agrawal B, et al. 1998. Cancer Res. 58:4079. 3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386. |
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| Gene ID | 4582 |
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| UniProt | View information about CD227 on UniProt.org |
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| Clone | 16A |
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| Regulatory Status | RUO |
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| Other Names | Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6 |
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| Isotype | Mouse IgG1, λ |
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