Beckman Coulter, 355619, 25 mm Diameter Cap Assembly Aluminum Bottle, Quantity of Six
Catalog Number: 355619
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Jurkat cells transfected with MUC1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1. |
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| Application References(PubMed link indicates BioLegend citation) | Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed |
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| RRID | AB_3083341 (BioLegend Cat. No. 355619) AB_3083341 (BioLegend Cat. No. 355620) |
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| Structure | 500-1000 kD proteoglycan (glycosylated form) |
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| Distribution | Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers |
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| Interaction | Complexes with SOS1 and Grb2 in RAS signaling |
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| Ligand/Receptor | ICAM-1 and Her2/neu (ErbB2) |
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| Cell Type | Dendritic cells, Epithelial cells, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339. 2. Agrawal B, et al. 1998. Cancer Res. 58:4079. 3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386. |
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| Gene ID | 4582 |
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| UniProt | View information about CD227 on UniProt.org |
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| Clone | 16A |
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| Regulatory Status | RUO |
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| Other Names | Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6 |
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| Isotype | Mouse IgG1, λ |
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