Beckman Coulter, 357325, 2 mL Quick-Seal® Polypropylene Tube Kit (for NVT 100), 13 x 25mm - 50Pk
Catalog Number: 357325
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Pack Size2 mL
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Neuroblastoma cell line LAN-1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a. |
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| Application References(PubMed link indicates BioLegend citation) | Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt) Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt) Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC) |
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| RRID | AB_2941536 (BioLegend Cat. No. 357325) AB_2941536 (BioLegend Cat. No. 357326) |
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| Distribution | Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma |
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| Function | Cell attachment to extracellular matrix |
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| Cell Type | Mesenchymal cells, Neurons |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | 1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260. 2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740. 3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200. |
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| Gene ID | 2583 |
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| UniProt | View information about Ganglioside GD2 on UniProt.org |
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| Clone | 14G2a |
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| Regulatory Status | RUO |
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| Other Names | Disialoganglioside GD2, Ganglioside G2 |
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| Isotype | Mouse IgG2a, κ |
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