Beckman Coulter, 358615, 7 x 20mm Delrin Tube Adapter, Quantity of Four
Catalog Number: 358615
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | LOX-1 ectodomain: Fc fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA |
| Preparation | The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA |
| Application | PG - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions. Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunofluorescent staining of acetone-fixed frozen human skin sections1. |
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| Additional Product Notes | TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq. The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation. View more applications data for this product in our Scientific Poster Library. |
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| Application References(PubMed link indicates BioLegend citation) | Li D, et al. 2012. J. Exp. Med. 209:109. (IHC) |
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| RRID | AB_2890788 (BioLegend Cat. No. 358615) |
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| Structure | Single pass, type II, transmembrane protein, 31 kD, homodimer, member of the C-type lectin superfamily. |
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| Distribution | Endothelial cells, smooth muscle, platelets, fibroblasts, macrophages; Upregulated by inflammatory and oxidative stimuli |
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| Function | Scavenger receptor, involved in endocytosis, phagocytosis and cytokine production |
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| Ligand/Receptor | Recognizes numerous ligands including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, oxidized LDL |
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| Cell Type | Dendritic cells, Endothelial cells, Fibroblasts, Macrophages, Platelets |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | 1. Yin Y, et al. 2013. Eur. J. Cell Biol. 92:150. 2. Khaidakov M. and Mehta JL. 2012. PLoS One. 7:e46973. 3. Sawamura T, et al. 2012. Curr. Opin. Lipidol. 23:439. 4. Lin FY, et al. 2011. J. Immunol. 186:4405. 5. Parlato S, et al. 2010. Blood 115:1554. |
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| Gene ID | 4973 |
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| UniProt | View information about LOX-1 on UniProt.org |
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| Clone | 15C4 |
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| Regulatory Status | RUO |
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| Other Names | OLR1, LOX1, LOXIN, SLOX1, CLEC8A, SCARE1 |
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| Isotype | Mouse IgG2a, κ |
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| Barcode Sequence | ACCCTTTACCGAATA |
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