Send an RFQ for Beckman Coulter 717254, Biomek Tips P20 Non-Sterile. Iright supports global sourcing and international shipment for life science and laboratory products.
| Source | Human Vitronectin, amino acids Asp20-Leu478 (Accession # P04004), with a N-terminal 9His tag, was expressed in 293E cells. |
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| Molecular Mass | The 482 amino acid recombinant protein has a predicted molecular mass of approximately 54.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 70-80 kD in SDS-PAGE. |
| N-terminalSequence Analysis | The predicted N-terminal amino acid is Ala. |
| Purity | > 95%, as determined by Coomassie stained SDS-PAGE. |
| Formulation | 0.22 µm filtered protein solution is in PBS, pH 7.2 |
| Endotoxin Level | Less than 0.1 EU per μg of protein as determined by the LAL method |
| Concentration | 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. |
| Storage & Handling |
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| Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Activity | Recombinant human vitronectin induces B16F1 cell adhesion in a dose-dependent manner. ED50 of this effect is 0.025 - 0.25 µg/mL. |
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| Application | Bioassay |
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| Application Notes | BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected]. |
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| Structure | Monomer |
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| Distribution | Vitronectin is synthesized primarily in the liver, but it is also expressed in retina, brain and vascular smooth muscle cells. |
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| Function | Role in cell adhesion, cell spreading, cell migration, bone remodeling and immunity. Vitronectin is a complement regulatory component. |
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| Interaction | Interacts with most cell types expressing integrin receptor |
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| Ligand/Receptor | An 'Arg-Gly-Asp' (RGD) sequence of vitronectin binds alphav beta3, alphav beta5, alphav beta1, alpha IIb beta3, alphav beta6, and alphav beta8 integrins, plasminogen activator inhibitor-1 (PAI 1) and urokinase receptor |
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| Bioactivity | Recombinant human vitronectin induces B16F1 cell adhesion in a dose-dependent manner. ED50 of this effect is 0.025-0.25 µg/mL. |
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| Cell Type | Endothelial cells, Epithelial cells, Fibroblasts |
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| Biology Area | Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Neurodegeneration, Signal Transduction |
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| Molecular Family | Adhesion Molecules |
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| Antigen References | Preissner KT, et al. 1998. Thromb. Res. 89:1-21. Tollefsen DM, et al. 1990. J Biol Chem. 265:9778-9781. Chillakuri CR, et al. 2010. FEBS Lett. 584: 3287-3291. Harburger DS, et al. 2009. J. Cell Sci. 122:159-163. Braam S, et al. 2008. Stem Cells. 26:2257-2265. Takagi J, et al. 2002. Cell. 110:599-611. Martínez-Morales JR, et al. 1997. Development. 124:5139-5147. Cheresh DA, et al. 1989. Cell. 57:59-69. Wu CC, et al. 2018. Biomaterials. 185:142-154. |
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| Gene ID | 7448 |
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| UniProt | View information about Vitronectin on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | VN, V75, VNT, Complement S-protein, Serum Spreading Factor,Somatomedin B, Complement S-protein |
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