Beckman Coulter, B31167, PerFix-nc Cell Permeabilization Kit, 75 Tests, CE-IVD

The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation.
Accurate detection of both intracellular and extracellular epitopes are obtained, while:
• There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
• Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
• Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
• Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.