Description
Use our combined iScript RNase H + reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
Key Features and Benefits
• Sensitive and reproducible singleplex or multiplex reactions directly from RNA
• Advanced formulation for amplifying difficult target sequences and challenging samples
• Tested with ethanol for inhibitor tolerance and compatible with up to 4 µl crude lysate
• Works on any real-time PCR system
Kit Components
• Reverse transcriptase — 2.5 ml (1 x 2.5 ml)
• Reaction mix — 50 ml (1 x 50 ml)
• Nuclease-free water — 45 ml (1 x 45 ml)
Instrument Compatibility
iTaq Universal Probes One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq Universal Probes One-Step Kit
• qPCR/real-time PCR
• Gene expression analysis
• Multiplexing
• Absolute quantification
• Mutation detection
• Pathogen detection
• Viral detection (load)
• Genetically modified organisms (GMO) characterization
• Genetic profiling
Packaging Options
The iTaq universal probes one-step kit is also available in a format of 100 reactions (1725140) and 500 reactions (1725141).
Related Products
Bio-Rad has also developed one-step kits for SYBR ® Green specific applications.
