Description
The ESR1 gene encodes estrogen receptor (ER) subtype alpha. The ddPLEX ESR1 Mutation Detection Assay quantitatively interrogates seven key ESR1 variants across three exons: exon 5 (E380Q), exon 7 (S463P) and exon 8 (L536R, Y537C, Y537S, Y537N and D538G), and their wild-type counterparts.
The assay consists of sufficient reagents for 120 reactions including 60 reactions of each for the Mutant Assay and Wild-Type Assay.
The assay used with the Bio-Rad QX600™ Droplet Digital™ PCR System or QX600™ AutoDG Droplet Digital™ PCR System and is compatible with QX Manager Standard Edition and Premium Edition software for data analysis.
This assay is a component of the ddPLEX ESR1 Mutation Detection Kit (17009501).
Features & Benefits
• Highly sensitive (0.01%–0.025% VAF*) single-well discrimination of seven key ESR1 gene mutations
• Same-day results with a streamlined workflow
• Flexible sample input of DNA from plasma or FFPE
• Kit includes positive and internal controls
• Sensitivity increased with patented wild-type suppression chemistry
• ddPCR-powered reproducibility and specificity using the QX600 ddPCR system and QX Manager Software
* Variant Allele Frequency
Applications & Uses
• For the detection, discrimination and quantification of key ESR1 mutations for breast cancer research, from cfDNA in plasma and/or DNA in formalin-fixed paraffin embedded (FFPE) tissue samples.
Product Contents
The ddPLEX ESR1 Mutation Detection Assay includes the following components for 120 reactions and can accommodate up to 42 samples plus controls. Additional reagents are needed, please see the ddPLEX ESR1 Mutation Detection Kit (17009501):
Configuration Options
ddPLEX ESR1 Mutation Detection Kit
Other Components from the ddPLEX Mutation Detection Kit
The ddPLEX ESR1 Mutation Detection Assay should be used with the QX600 ddPCR System and either the Manual Droplet Generator or the Automated Droplet Generator. Equipment, key consumables, and reagents necessary for the QX600 System are listed below (additional items may be needed, please see User Guide for more details)
Key Droplet Generation & Reader Equipment, Consumables & Reagents
Reagents & Consumables for Automated Droplet Generation Only
Reagents & Consumables for Manual Droplet Generation Only
Mutations Detected by ddPCR Multiplex Mutation Screening Kits
*Assays for each of the mutations detected by these kits can also be ordered individually. Each kit contains 20x ddPCR Mutation Screening Multiplex Assay (1 x 200 μl) and 2x ddPCR Supermix for Probes (No dUTP) (2 x 1 ml).
**Each kit contains ddPCR Multiplex Supermix (2x 600 ul), ddPCR MSI Assay 1 (BAT25/26), ddPCR MSI Assay 2 ((NR21/24), ddPCR Assay 3 (Mono27), Uracil-DNA Glycosylase (UDG), positive control, and nuclease-free water.
Software Compatibility
The ddPLEX ESR1 Mutation Detection Kit is compatible with the following QX Software selections:
Related Products
• ddPCR Microsatellite Instability (MSI) Kit
• BRAF V600 ddPCR Multiplex Mutation Screening Kit
• KRAS ddPCR Multiplex Mutation Screening Kits
• NRAS ddPCR Multiplex Mutation Screening Kits
• EGFR Exon19 Deletions ddPCR Multiplex Mutation Kit
Related Category
• Digital PCR Assays
Supporting Documents
• ddPLEX ESR1 Mutation Detection Kit, User Guide
• ddPLEX ESR1 Mutation Detection Kit, Flyer
For Research Use Only. Not for use in diagnostic procedures.
