Description
Use the Cloning and Sequencing Explorer series to give your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.
The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:
• Extract genomic DNA from plants
• Amplify a GAPDH gene using PCR and analyze using electrophoresis
• Purify the PCR product
• Ligate the PCR product into a plasmid
• Transform the ligated plasmid into bacteria
• Grow minipreps of the transformed bacteria
• Isolate plasmid DNA and analyze by restriction digestion
• Sequence plasmid DNA (sequencing service not included)
• Analyze GAPDH gene sequence using bioinformatics
Features and Benefits
• 6–8 week series of laboratory activities made up of eight modular kits
• Provides sufficient materials for 12 student workstations, up to four students per workstation
• Sequencing service not included
For more information and resources, visit the Cloning and Sequencing Explorer Series product page.
Related Products
• Nucleic Acid Extraction Module
• GAPDH PCR Module
• Electrophoresis Module
• PCR Kleen™ Spin Purification Module
• Ligation and Transformation Module
• Microbial Culturing Module
• Aurum™ Plasmid Mini Purification Module
• Sequencing and Bioinformatics Module
Supporting Documents
• Bio-Rad Explorer™ Cloning and Sequencing Explorer Series Curriculum Manual
