Description
Use the ddPCR E. coli Residual DNA Quantification Kit to detect femtogram amounts of residual E. coli host cell DNA (HCD) in various types of bioproduction intermediates.
To ensure product quality and safety of biopharmaceutical products, the amount of residual host cell DNA must meet guidelines established by regulatory agencies including the World Health Organization, the European Pharmacopeia, and the U.S. Food and Drug Administration.
Note: for research use only.
Kit Components
• 20x ddPCR E. coli Residual DNA Quantification Assay — 1 x 200 µl
• ddPCR Supermix for Residual DNA Quantification — 2 x 1 ml
Key Features and Benefits
• Direct sample input — eliminates the need for DNA extraction; partitioning minimizes the effects of inhibitors
• Highly sensitive, precise detection and quantification of HCD without a standard curve: LOD = 15 fg or better LOQ ≤30 fg per 20 µl reaction Range = 30 fg–30 pg
• Minimal method development — assay and supermix guaranteed free of detectable levels of CHO and E. coli DNA
• Accurate quantification even with highly fragmented DNA — kit detects 16s rRNA genes in fragments ≥200 bp
• Fast, simple workflow — completed within hours rather than days
• High sample throughput — allows processing of several hundred samples in a single day
Additional Equipment
This kit is for use with one or more of the following instruments and accessories:
• QX200 Droplet Digital PCR System
• QX200 AutoDG Droplet Digital PCR System
• PX1™ PCR Plate Sealer
• C1000 Touch™ Thermal Cycler with 96 Deep–Well Reaction Module
More Information
Learn more about the features of other Digital PCR Assays for copy number determination and mutation detection.
Documents
• ddPCR E. coli Residual DNA Quantification Kit Product Insert
