Description
Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.
• 0.5 ml is generally sufficient for 60 to 100 cell transfections in 35 mm plates
• Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
• Transfection at culture densities from 40% to 90%
• Designed to work in media containing serum but can be used in the absence of serum
• No post transfection media changes required
• Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene
The Transfection Protocol Online Library contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The TransFectin Lipid Reagent Instruction Manual provides information about optimization as well as changes in protocols when changing from a different cationic lipid.
Packaging Options
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• SmartSpec™ Plus Spectrophotometer
• Fixable Viability Dyes
Related Categories
• Electroporation
• Biolistic Particle Delivery Systems
• Nucleic Acid Extraction & Purification
• PCR Amplification
Supporting Documentation
• TransFectin Lipid Reagent Brochure
• TransFectin Lipid Reagent Instruction Manual
• Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types Bulletin
• Transfection Reagent References
Online Applications
Transfection
