Bio-Rad, 1725095, SingleShot™ SYBR<sup>®</sup> Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

Name: SingleShot™ SYBR ® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions
Brand: Bio-Rad
SKU: 1725095

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR ® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions, SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

Catalog Number: 1725095

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Category

One-Step RT-PCR Kits

Pack Size100 x 50 µL

TagsReagents Research Products Life Science Products LSG GXB - Gene Expression Amplification>Real-time PCR Reagents PCR & qPCR PCR Reagents & qPCR Reagents Specialty Reagents 1-Step RT-qPCR from RNA or Cells Lysis Kits Cell Lysis RT-qPCR Kits 100 x 50 µl reactions

Description

Use the SingleShot™ SYBR ® Green One-Step Kit to obtain high-quality RT-qPCR data with SYBR ® Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

• Cell lysate from 10 to 100,000 cells ready in 20 minutes

• Complete removal of genomic DNA while preserving RNA integrity

• Optimal accuracy and linear dynamic range of qPCR data across genes

• Results comparable to using purified RNA, including dynamic range

• Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )

• SingleShot RNA Control ensures optimal cell and lysate inputs

Optimized Sample Input

The SingleShot™ SYBR ® Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:

Kit Contents

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR ® Green One-Step Kit

The SingleShot™ SYBR ® Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Related Products

The SingleShot™ SYBR ® Green One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-pPCR Kits:

• SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR ® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )

• SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR ® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )

• SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
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