Description
Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.
• Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
• Read wavelengths, 650–750 nm
• Absorbance values are stable ≥ 1 h
• Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
• Determine protein recovery in cell lysates
Sample components compatible with the RC DC Protein Assay include:
• Ammonium sulfate, 0.5 M β-mercaptoethanol, 10%* Brij 35, 1% C12E8, 0.2% Calcium chloride, 50 mM CHAPS, 2% CHAPSO 1%
• Dithiothreitol (DTT), 350 mM* EDTA 0.1 M Guanidine hydrochloride, 0.4 M Imidazole, 0.5 M Laemmli buffer (5% β-mercaptoethanol) Nonidet P-40, 2% Octyl-β-glucoside, 1%
• ReadyPrep™ Sequential Extraction Reagents 2 and 3* SDS, 10% Tributylphosphine (TBP), 2 mM Tris, pH 8.4, 0.5 M Triton X-100, 2% Tween 20, 2% Urea, 4 M
* Two washes required for highest concentrations
Product Contents
• RC Reagent I, 250 ml
• RC Reagent II, 250 ml
• Alkaline copper tartrate, 250 ml
• Dilute Folin reagent, 2 L
• Surfactant solution, 5 ml
• Lyophilized bovine γ-globulin, 1 vial
Packaging Options
Related Products
• Protein Standard I, lyophilized bovine γ-globulin ( 5000005 )
• Protein Standard II, lyophilized bovine serum albumin ( 5000007 )
• Semimicrovolume Disposable Polystyrene Cuvettes ( 2239955 )
• Standard Disposable Polystyrene Cuvettes ( 2239950 )
• Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate ( 5000113 )
• Protein Assay Reagent B, refill, 1 L Folin reagent ( 5000114 )
• Protein Assay Reagent S, refill, 5 ml surfactant ( 5000115 )
• RC Reagent I, refill, 250 ml ( 5000117 )
• RC Reagent II, refill, 250 ml ( 5000118 )
Related Categories
• SmartSpec™ Plus Spectrophotometer
• Microplate Readers
• Mixing Devices
• Centrifuges
• Protein Assay Kits and Cuvettes
Supporting Documentation
• RC DC Protein Assay Product Information Sheet
• RC DC Protein Assay Instruction Manual
• Colorimetric Protein Assays Application Note
Reference
Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713
