Bio-Rad, AHP1799, NogoA antibody

Rabbit anti Human NOGOA antibody recognizes human Reticulon-4 (RTN4), also known as NogoA, neurite outgrowth inhibitor, Foocen, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, Reticulon-5 or RTN-x. NogoA is a 1192 amino acid multi pass transmembrane protein associated with the endoplasmic reticulum, a member of a family of integral membrane proteins termed reticulons. Six isoforms of NogoA can be generated by alternative splicing, the canonical isoform 1 is predominantly expressed in the brain and testis with weaker expression in the heart and skeletal muscle. Reticulons are involved in various neurodegenerative diseases such as Amyotrophic lateral sclerosis,and multiple sclerosis ( Chiurchiù et al. 2014 ). Reticulon proteins have been demonstrated to regulate many cellular processes and interact with multiple proteins and receptors such as BACE . NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor ( Niederöst et al. 2002 ). NogoA is highly expressed in oligodendrocytes in the white matter of the CNS ( Kuhlmann et al. 2008 ). Blocking NogoA activity with antibodies or other factors results in improved long distance axonal regeneration and functional recovery in experimental CNS lesion models ( Schwab 2004 ). NOGOA has a predicted molecular weight of 130 kDa however, despite its predicted molecular weight, NogoA typically migrates at ~180 kDa in an SDS-PAGE. Rabbit anti human NOGOA antibody is expected to recognize all isoforms of NogoA.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.