Bio-Rad, AHP288, NFkB p65 antibody

Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFκB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFκB p65 is a 551 amino acid ~65 kDa subunit of the NFκB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFκB appears to be the p65-p50 heterodimer. In the active form NFκB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFκB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IκB ( UniProt: Q04206 ) In a gel supershift assay Rabbit anti Human NFκB p65 antibody was found to be active against all p65 containing human, mouse or rat NFκB complexes using 0.5ul to 1.0ul per assay.

Storage Information
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.